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Thermodynamic parameters of TraI binding to dsDNA oligomers of length ranging from 9 to 27 bp in buffer A at 25 °C
The following abbreviations are used: n, maximum stoichiometry; Kint, intrinsic binding constant; ω, cooperativity parameter; NA, not applicable.
| dsDNA9 | dsDNA11 | DsDNA15 | dsDNA19 | DsDNA23 | dsDNA27 | |
|---|---|---|---|---|---|---|
| n | 1 | 1 | 1 | 2 | 2 | 2 |
| Kint ×105 (m−1) | 9.9 ± 7.9 | 4.7 ± 0.2 | 5.1 ± 0.2 | 5.2 ± 0.5 | 6.2 ± 0.3 | 5.4 ± 0.4 |
| ω | NA | NA | NA | 18.1 ± 3.8 | 38.6 ± 7.7 | 14.8 ± 2.2 |
