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AlF4− response of wild-type αT* and αT* (S43N). Wild-type αT* (red) and αT*(S43N) (green) were incubated in HMDM buffer. At the indicated time, 20 μl of a premixed aliquot containing 250 mm NaF and 150 μm AlCl3 to generate AlF4− was added and the enhancement in tryptophan fluorescence (excitation: 280 nm; emission: 340 nm) was monitored.
