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. 2011 Feb 1;286(14):12712–12723. doi: 10.1074/jbc.M110.189605

FIGURE 3.

FIGURE 3.

PI3K is required for plasma membrane targeting of RasGRP1 in response to hydrogen peroxide or PDGF. A, DT40 cells expressing GFP-tagged TAPP1 PH domain or RG1 were either untreated (nil) or pretreated for 15 min with LY303511 (LY30), LY294002 (LY29), or wortmannin (Wm) and then stimulated with H2O2 in the presence of LY303511, LY294002, or wortmannin for 10 min. Scale bar, 5 μm. B, NIH 3T3 cells expressing GFP-tagged PH domains of TAPP1, Btk, or RG1 were untreated (nil) or pretreated for 15 min with LY303511, LY294002, or wortmannin prior to stimulation with PDGF-BB in the presence of LY303511, LY294002, or wortmannin for 5 min. Scale bar, 10 μm.