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Constitutive plasma membrane localization of RasGRP1 in WEHI-231 B cells is mediated by PI3K and the BHC. A, unstimulated WEHI 231 cells expressing GFP-tagged RG1 or the indicated mutants. Scale bar, 5 μm. B, unstimulated WEHI 231 cells expressing GFP-tagged RG1 were treated with LY294002 (LY29) to inhibit PI3K or with LY303511 (LY30) as a control for 30 min. Scale bar, 5 μm.
