TABLE 1.

BHC within the PT domain mediates BCR-induced plasma membrane targeting of RasGRP1

	% of cells with detectable signal at pma		Signal intensity, pm/cytoplasmb
	Nil	+αIgM	+αIgM
RG1	0	100	7.7 ± 2.7c
RG1ΔPT	0	0	1.0 ± 0.1
RG1ΔLZ	0	95	3.3 ± 0.9
PT-C	19	20	1.1 ± 0.1
BHC	0	90	2.4 ± 0.5
RG1-Wμ	0	35	1.4 ± 0.3
RG1-FμWμ	0	10	1.1 ± 0.1
RG1-RKR/Kμ	0	30	1.2 ± 0.2

^a 200+ DT40 cells were scored for detectable concentration of GFP signal at the plasma membrane.

^b Signal intensities at plasma membrane and cytoplasm were determined as described under “Experimental Procedures.”

^c Values are the mean ± S.D.