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High efficiency of virus infection leads to high receptor expression. Primary cultures of hippocampal neurons from wild-type or β-arrestin2−/− mice were infected with AdOPRM1 or AdS375A for 3 days. The receptor expression was determined with binding assay after infection (A). The efficiency of the infection was indicated by the percentage of HA-positive (green in C) cells in DAPI-positive (blue in C) cells (B and C).
