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Comparison of catalytic turnover of succinate-oxidase and fumarate-reductase reactions catalyzed by isolated wild-type QFR and FrdB T205H mutant
| Succinate-K3Fe(CN)6 |
Succinate-UQ2 |
MQ1H2-fumarate |
||||
|---|---|---|---|---|---|---|
| WT | T205H | WT | T205H | WT | T205H | |
| Turnover rates (s−1) | ||||||
| pH 6.0 | 3.2 | 3.5 | 1.9 | 1.7 | 144 | 64 |
| pH 7.0 | 7 | 7.6 | 7.6 | 4.2 | 220 | 121 |
| pH 8.0 | 14.6 | 15 | 12 | 2.8 | 177 | 119 |
| Km values (μm), pH 7.0 | ||||||
| KmUQ2 | 2 | 8.5 | ||||
| KmMQ1H2 | 4 | 22 | ||||
