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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Biochemistry. 2011 Feb 22;50(11):1940–1949. doi: 10.1021/bi101606e

FIGURE 4.

FIGURE 4

Effects of Asp-N-cleaved activated Rho (Asp-NRho*) on peptide C phosphorylation by GRK1535H6. Both the wt protein (■) and the cross-linked T8C/N480C mutant (□) show activation by Asp-N Rho* in a concentration-dependent fashion, while the ΔN19 mutant did not show activation by Asp-NRho* (●). Data were fitted to eq 1 from four independent experiments. Ymin, Ymax, and KD,app values are (79 ± 5) × 10−6 s−1, (780 ± 80) × 10−6 s−1, and 12 ± 5 µM for wt and (370 ± 40) × 10−6 s−1, (1900 ± 170) × 10−6 s−1, and 15 ± 6 µM for the cross-linked T8C/N480C mutant, respectively.