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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Genesis. 2010 Nov 17;48(12):693–706. doi: 10.1002/dvg.20679

Fig. 7. Af17 is expressed in the neurons of adult animals.

Fig. 7

A. X-gal staining of a sagittal brain section from an Af17-/- animal with stained cells in the cerebral cortex and the CA areas of the hippocampus. B & B’. High magnification images of the boxed areas in A. Note the staining in the CA areas but not in the dental gyrus. C. X-gal staining of a sagittal brain section from an Af17-/- mouse showing stained cells in the cerebellum. GL: granular layer; ML: molecular layer. D. A high magnification image of the boxed area in C. Sections in A-D were counterstained with nuclear fast red. E. Immunohistochemical (IHC) staining for GFAP in a transverse brain section after X-gal staining. Note that the blue X-gal staining and the brown IHC staining do not coexist in the same cells. F. A higher magnification image of the boxed area in E. G-J. IHC staining for NeuN and X-gal staining in a transverse brain section showing the hippocampal CA areas (G) and the cerebral cortex (I), with higher magnification images of their boxed areas shown in H and I, respectively. Note the cells stained by both X-gal and IHC (arrows). K-N. Hematoxylin & Eosin staining of sagittal brain sections from 6-8 week old WT (K & M) and Af17-/- (L & N) animals showing similar morphology of the hippocampus (K & L) and the cerebellum (M & N). All animals shown were 6 weeks old.