Fig. 1. IRF3-deficiency protects against alcohol-induced liver damage.
Wild-type (WT) and IRF3-deficient (IRF3-KO) were fed Lieber DeCarli ethanol or control (pair-fed) diet and sacrificed after 4 weeks. Livers were fixed in formalin and stained with H&E or with Oil-red-O; magnification 200x (A). Serum ALT levels (B) were analyzed. Messenger RNA levels of liver (C) tumor necrosis factor α (TNFA), (D) pro-interleukin 1-beta (IL-1β) and (E) interferon stimulated gene ISG56 were analyzed by real-time PCR and normalized to 18s.Values are shown as mean ± SEM fold increase over WT pair-fed control group (9-12 mice per group). Numbers in graphs denote p values; *) p < 0.05 vs. pair-fed WT mice; #) p < 0.05 vs. ethanol-fed WT mice.