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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Hepatology. 2011 Jan 10;53(2):649–660. doi: 10.1002/hep.24059

Fig. 6. Type I IFN-dependent induction of IL-10 modulates inflammatory cytokines in mononuclear cells.

Fig. 6

Murine RAW264.7 macrophages were stimulated with 100 ng/mL LPS, 1000 IU/mL murine recombinant IFNα2a, 10 ng/mL recombinant murine IL-10 and 1 μg/mL anti-mouse IL10 receptor antibody (anti IL-10R Ab). Human peripheral blood mononuclear cells (PBMCs, N=4) were stimulated with 10 ng/mL LPS, 1000 IU/mL human recombinant IFNα2, 10-2000 pg/mL recombinant human IL-10 and 1 μg/mL anti-human IL10 receptor antibody (anti IL-10R Ab). Protein levels of (A,B) interleukin 10 (IL-10), (C,D,E) tumor necrosis factor α (TNF-α) and (D,F) interleukin 1-β (IL-1β) were analyzed using ELISA. Values are shown as mean ± SEM. Numbers in graphs denote p values; *,#,§,†) p < 0.05 vs. respective control group