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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cell Cycle. 2008 Sep 15;7(18):2846–2855. doi: 10.4161/cc.7.18.6627

Figure 2.

Figure 2

Custom antibodies to phosphorylated motifs of ATM (S385), CDK2 (T397) and p70s6K (S466) kinase recognize site-specific phosphorylation of ΔNp63α in HNSCC 029 cells upon cisplatin exposure. HNSCC 029 cells (105) were treated with 10 μg/ml cisplatin for the indicated time periods in the presence of 20 μM MG-132 (26S proteasome inhibitor). Total lysates resolved by SDS-PAGE were immunoblotted with the indicated antibodies. Protein levels were normalized with antibody to β-actin.