. Author manuscript; available in PMC: 2011 Apr 1.

Published in final edited form as: Cell Cycle. 2008 Sep 15;7(18):2846–2855. doi: 10.4161/cc.7.18.6627

Table 2.

ΔNp63α phosphorylation sites identified by mass spectrometry

Observed proteolytic	Residue	Kinase	Mascot	Xcorr
phosphopeptide¹			Score²	Value³
Chymotrypsin: VSQLINPQQRNAL (384–396)	S385	ATM	41	2.21
Chymotrypsin: LTPTTIPDGM (396–405)	T397	CDK2	43	2.37
Plus Proteinase K (396 397 399 400 401)
Chymotrypsin: LGCSSCL (462–468)	S466	p70s6K	44	2.53
Plus NTCB: GCS (463–466)	S466	p70s6K	49	3.23

All phosphopeptides were confirmed by tandem mass spectrometry. NTCB (2-nitro-5-thiocyanobenzoic acid).

Mascot scores of individual peptides were determined as described elsewhere.⁶²

SEQUEST Xcorr value of individual peptides were determined as described elsewhere.⁴⁹