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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Methods. 2008 Oct 16;46(3):177–182. doi: 10.1016/j.ymeth.2008.09.014

Figure 2. Activation of Ca2+ release via transiently expressed M3 muscarinic Receptor.

Figure 2

In A, concentration vs Response relationship for an individual DT40-3KO cell expressing S2-InsP3R-1 and the M3 muscarinic receptor. In B, a similar experiment is performed in a cell expressing a construct harboring phosphomimetic charge mutations (“EE” InsP3R-1). C shows pooled data where the magnitude of the initial peak of a particular response is normalized to the maximal peak in an individual cell. Adapted from Ref [31], with permission.