Figure 10.

LepRb neurons are synaptically coupled with neurons in the rRPa. In LepRb^EGFP reporter mice, LepRb^EGFP-positive fibers (green) are seen in close proximity to neurons in the rRPa (red) (B, n = 10). The same section is shown as an overview with NeuN stain in A. Transsynaptically labeled WGA-positive neurons are found in the rRPa of LepRb^WGA mice (D, n = 5), indicating synaptic coupling of LepRb neurons with rRPa neurons. An overview of the same sections is shown in C. 4V, Fourth ventricle; py, pyramidal tract.