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. Author manuscript; available in PMC: 2012 Apr 12.
Published in final edited form as: Brain Res. 2011 Apr 12;1384:15–22. doi: 10.1016/j.brainres.2011.02.014

Figure 2. Tolerance to prolonged fasting in mice with Glut3+/− genotype.

Figure 2

Mice at 10-12 weeks of age were divided into four groups of four animals, male Glut3+/−, female Glut3+/−, male Glut3+/+, and female Glut3+/+. After ad lib feeding overnight, food was removed and glucose was determined in blood from a cheek puncture at times 0, 6 hr, 12 hr, 24 hr, and 48 hrs. Each mouse had single blood glucose determinations at five different time separated by one week before the next sequentially increased fasting time period. Ambient temperature was maintained at 29°C to minimize torpor that can occur with food restriction. At no time was there evidence of seizure activity or other change in behavior among the mice during the 48 hours of frequent observation. No significant difference in glucose concentrations was found comparing the groups at any of the durations of fasting.