Figure 3. Uptake of glucose into brains of mice with Glut3+/− genotype.

Ten mice (5 males, 5 females) were injected with 1 μCi of ³H-2-deoxy glucose via tail vein and 45 minutes later were sacrificed by CO₂ inhalation and brain removal. Eleven littermate controls (5 males, 6 females) were similarly evaluated. All animals were allowed free access to food overnight, but food was removed two hours prior to sacrifice. Each brain was removed, rinsed in saline, weighed, and dissolved in Scintigest (Fisher Scientific) at 50°C for at least 18 hours. Brain uptake of glucose was quantified as cpm ³H per brain. Male brains were slightly larger, but there was no difference between the Glut3+/− and Glut3+/+ groups, whether the data were expressed as cpm/brain or cpm/mg brain.