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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: J Mol Endocrinol. 2011 Feb 15;46(2):67–79. doi: 10.1677/JME-10-0110

Figure 1.

Figure 1

Mdm2 knockdown inhibits estrogen-induced ERα and ERα/Sp-mediated transactivation. MCF-7 cells were transfected with pERE3 (A) or pSp13 (B) and ZR-75 cells were transfected with pERE3 (C) or pSp13 (D) andand various (or no) oligonucleotides and treated with DMSO or 10 nM E2. Luciferase activity was determined as described in the Materials and Methods. Significant (p < 0.05) induction by E2 (*) or inhibition by iMdm2 or iGL2 (**) are indicated. Results are expressed as means ± SE for at least 3 separate determinations for each treatment group. Minimal activity was observed in cells transfected with the empty vector, and treatment with DMSO or E2 did not induce activity. (E) Mdm2 knockdown. MCF-7 cells were transfected with a non-specific (siCT) or siMdm2 oligonucleotide, and whole cell lysates were analyzed by western blots as described in the Materials and Methods. MCF-7 cells were used for whole cell lysate analysis due to higher transfection efficiency compared to ZR-75 cells.