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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Cancer Res. 2010 Dec 14;9(2):195–202. doi: 10.1158/1541-7786.MCR-10-0363

Figure 6.

Figure 6

GT-094 modulates miR-27a and ZBTB10 expression. (A) GT-094 decreases miR-27a expression in RKO and SW480 cells. Cells were treated with 20 or 50 μmol/L GT-094 and 5 mmol/L GSH alone or in combination for 24 h, and miR-27a was determined by real time PCR as outlined in the Materials and Methods. (B) GT-094 induces ZBTB10 in RKO and SW480 cells. Cells were treated with 20 or 50 μmol/L GT-094 and 5 mmol/L GSH alone or in combination for 24 h, and ZBTB10 mRNA levels were determined by real time PCR as outlined in the Materials and Methods. Significant (p<0.05) inhibition of miR-27a and induction of ZBTB10 (*) and inhibition of these responses by GSH (**) are indicated. (C) Proposed molecular mechanism for GT-094-induced ROS and ROS-dependent disruption of the miR-27a:ZBTB10-Sp/Sp-regulated gene axis.