Figure 1.

Decorin suppresses Sema3A messenger RNA expression in adult cerebral cortex injuries. Graphs show results of quantitative PCR analysis of Sema3A messenger RNA expression of tissue samples from cortical injuries infused with either human recombinant decorin (2.5 mg/ml) or saline vehicle using Alzet mini-osmotic pumps for 8 days at a flow rate of 1 µl/h. Quantitative analysis was conducted using the comparative (2^ΔΔCt) method with samples from saline-treated tissues serving as reference calibrators. Data are represented as relative quantitation of Sema3A expression compared with saline-treated control samples that were given a reference value of 1. Analysis revealed a robust 66.0% (±5.0) reduction in Sema3A expression in decorin-treated injuries compared with saline-treated controls. All quantitative PCR reactions were run in triplicate and at least three fully independent experiments were conducted. Statistical analyses were made using the randomization test of the REST 2005 software. Error bars show standard deviations.