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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Reproduction. 2010 Feb 12;139(4):759–769. doi: 10.1530/REP-09-0518

Figure 1.

Figure 1

RT-PCR (A) and western blot (B) demonstrate that neonatal (D1), pubertal (D30) and adult (D60) gubernacula (Gub), similar to adult epididymis (Epi), contain Ar mRNA (A) and 110 kDa protein (B). Rpl19 and β-actin were used as loading controls for RT-PCR and western blot, respectively. Immunohistochemistry shows that AR protein was localized in the nuclei of gubernacular mesenchymal and cremaster muscle cells (D–F) and epididymal epithelium (C).Representative pictures show one of three mice in each age group. Mag. C–F=×150, Inset in F=×600.