Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Reproduction. 2010 Feb 12;139(4):759–769. doi: 10.1530/REP-09-0518

Figure 11.

Figure 11

Effects of DHT and INSL3 on cell differentiation in the primary cultures of mesenchymal cells isolated from the gubernacula of neonatal WT mice. Immunofluorescent staining of a mesenchymal cell marker, CD44 (A–E) and a muscle cell marker, troponin (F–J) showed that the combined treatment of DHT and INSL3 led to a reduction of CD44 (C) and appearance of troponin (H). Addition of either flutamide (Flut) or INSL3i abolished DHT and INSL3 effects (D, E, I and J). The nuclei are labeled with blue color by Hochest dye. Representative pictures show one of three independent experiments, and the gubernacular tissues used for the preparation of mesenchymal cells in each experiment were pooled from three mice. Mag. All=×300.