Figure 5.

Programmed death 1 PD-1)–dependent expression of cytokine inducible SH2-containing protein (CISH). A, CD4⁺CD25⁻ cells from healthy tuberculin reactors cultured with autologous monocytes (Mφ) in medium alone or with γ-irradiated Mycobacterium tuberculosis (M. tb; 10 μg/mL), with or without anti-PD-1 or isotype control antibody (10 μg/mL). After 4 d, whole-cell lysates were prepared and subjected to Western blot analysis with an antibody to CISH (upper panel). The blot was stripped and reprobed with an antibody to β-actin (lower panel). The result is representative of experiments performed on samples from 5 donors. Lane 1, Unstimulated cells; lane 2, M. tuberculosis–stimulated cells; lane 3, M. tuberculosis–stimulated cells with anti-PD-1; lane 4, M. tuberculosis–stimulated cells with isotype control antibody. B, CISH expression quantified by densitometry. Cells from 5 donors were prepared, and Western blot analysis was performed as detailed for panel A. Mean values and SEs are shown.