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. 2011 Mar 1;121(4):1585–1595. doi: 10.1172/JCI44630

Figure 5. Fgf8 rescues EMT defects in Six1/Eya1 mutant OFT explants.

Figure 5

(A–H) Six1–/–Eya1–/– mutants (E–H) had hypoplastic OFT cushions compared with WT littermate controls (A–D). Shown are serial histological sections of E11.5 outflow vessels proceeding from proximal (A and E) to distal (D and H). Brackets in A–D indicate WT OFT cushions, which were hypoplastic in the mutants (asterisks). Myo, myocardium. (I–K) The compound mutants (J and K) had fewer endothelial cells migrating from the OFT explant, undergoing EMT, and invading the gel than did WT controls (I) in cultured E9.5 OFTs. (L–N) The EMT defect was rescued by rFgf8b in the explant culture. Original magnification, ×230 (A–H); ×100 (I–N). (O and P) Quantification of results in I–N. White bars, without rFgf8b; gray bars, with rFgf8b. Student’s t test. n = 6 (control); 3 (Six1+/–Eya1–/– and Six1–/–Eya1–/–).