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. 2011 Mar 1;121(4):1349–1360. doi: 10.1172/JCI44855

Figure 6. Mitochondrial UPR regulation of NF-κB activity.

Figure 6

(A) LN229 cells transfected with control or CHOP-directed siRNA were treated with G-TPP and analyzed by immunoblotting. (B) siRNA-transfected LN229 cells as in A were treated with G-TPP and analyzed for NF-κB luciferase promoter activity, with or without TNF-α. Mean ± SD of replicates. *P = 0.018; ***P = 0.0007. (C) LN229 cells were transfected with the indicated plasmids and analyzed by immunoblotting. (D) Transfected LN229 cells as in C were analyzed for NF-κB luciferase promoter activity in the presence of TNF-α. Mean ± SD of replicates. *P = 0.02; **P = 0.0021. (E) LN229 cells transfected with pcDNA or a CHOP mutant lacking the leucine zipper (CHOP-ΔLeu) were analyzed for NF-κB luciferase promoter activity with or without TNF-α. Mean ± SD of replicates. ***P = 0.0006.