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. 2011 Mar 1;121(4):1349–1360. doi: 10.1172/JCI44855

Figure 7. Exploitation of mitochondrial UPR for combination anticancer therapy.

Figure 7

(A) Patient-derived (left) or cultured (right) glioblastoma cells were treated with TRAIL or G-TPP, alone or in combination, and analyzed by MTT. Mean ± SD of replicates. (B) The indicated cell types were treated as in A and analyzed for DNA content by PI staining and flow cytometry. The percentage of cells with hypodiploid (sub-G1) DNA content is indicated. Representative experiment. (C) U251 cells were labeled with TMRM, treated as indicated, and analyzed for loss of mitochondrial membrane potential after 0 or 5 hours by confocal laser microscopy. Representative experiment. Original magnification, ×600. (D) U251 cells treated as indicated were analyzed after 16 hours by immunoblotting. (E) Treated U251 cells were analyzed after 16 hours for annexin V/PI staining by flow cytometry. The percentage of cells in each quadrant is indicated. (F) Treated U87 cells were analyzed after the indicated time intervals by immunoblotting. Cl, cleaved.