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. 2011 Mar;178(3):1201–1209. doi: 10.1016/j.ajpath.2010.11.073

Figure 3.

Figure 3

Galectin-mRNA expression profile in CG treated paws of WT and Gal-1−/− mice. Real-time PCR analysis for Gal-1 (A), Gal-3 (B), and Gal-9 (C) mRNA levels were assessed in CG-treated paws of WT (black circle) and Gal-1−/− (white circle) mice collected 6, 24, 48, 96 hours post-CG, respectively. Data are expressed as mean ± SEM (eight mice per group). *P < 0.05, **P < 0.01, ***P < 0.001 as compared to control (WT Naïve). Immunohistochemistry was performed on CG-treated paws of WT (E, H) and Gal-1−/− (F, I) mice from the 96-hour time point to examine Gal-9 and Gal-1 expression in the tissue. WT treated with saline were used as controls (D, G). Images shown are representative of five mice per group with similar results. Scale bar, 150 μm. J: Cell-free inflammatory exudates recovered from CG treated paws of WT and Gal-1−/− mice, taken from the indicated time points (24, 48, and 96 hours), were analyzed by Western blotting to determine soluble Gal-1 and Gal-9 protein levels in the inflamed paw.