Table 1.
Marker | Time (hours) | Control (1 × 105/paw) | WT (1 × 105/paw) | Gal-1−/− (1 × 105/paw) |
---|---|---|---|---|
GR-1/CD11b | 48 | 0.29 ± 0.05 | 9.72 ± 1.43⁎† | 4.25 ± 1.23⁎ |
96 | 0.16 ± 0.02 | 0.53 ± 0.18 | 0.64 ± 0.10 | |
CD11c/CCR7 | 48 | 0.04 ± 0.01 | 1.21 ± 0.24⁎ | 0.74 ± 0.15⁎ |
96 | 0.07 ± 0.03 | 5.95 ± 1.64 | 5.17 ± 2.21 | |
F4/80 | 48 | 0.25 ± 0.02 | 3.7 ± 0.40⁎ | 2.31 ± 0.51⁎ |
96 | 0.22 ± 0.04 | 3.2 ± 1.41 | 2.29 ± 0.72 |
Flow cytometry was performed on cells recovered from CG treated paws of WT and Gal-1−/− mice at 48 hours (five mice per group) and 96 hours (three mice per group). Double and single staining was carried out for Gr1/CD11b (PMN), CD11c/CCR7 (migrating DC) and F4/80 (monocyte/ MØ). Data are expressed as mean ± SEM.
P < 0.05 as compared to WT naïve (control),
P < 0.05 as compared to Gal-1−/−.