Figure 10.

Neurofilament-positive neuronal inclusions in spinal cord were more frequent in Tg30xtau^−/− mice than in Tg30 mice. A, B: Nissl staining for hyaline inclusions in the anterior horn of the lumbar spinal cord in Tg30 (A) and Tg30xtau^−/− (B) mice. C: The density of inclusions in the anterior horn was significantly increased in Tg30xtau^−/− mice. ***P < 0.001 by Student's t-test (n = 5 mice for each genotype). D–E: Double immunolabeling of the anterior horn of Tg30xtau^−/− mouse with the PHF-1 tau antibody (D) and a neurofilament antibody (E). Most of the neuronal inclusions were positive for both phosphotau and neurofilament. Scale bar = 20 μm.