Table.
Stereological Parameters Used for Cell Counting
Area | Obj. | B (μm2) | H (μm) | Sampling grid (μm) | t (μm, mean ± SEM) | Σ OD | Σ CN | CE |
---|---|---|---|---|---|---|---|---|
Hippocampus | 100× | 900 | 9 | 125 × 90 | 19.9 ± 0.8 | 291 | 655 | 0.042 |
Spinal cord | 100× | 2500 | 12 | 150 × 125 | 20.0 ± 0.3 | 183 | 157 | 0.082 |
B and H, base and height of the optical disectors; CE, average predicted coefficient of error of the estimated total numbers of neurons; Obj., objective lens power; t, measured average section thickness after histological processing; Σ CN, average number of counted neurons per specimen; Σ OD, average number of optical disectors used per specimen. For methodology, see Schmitz and Hof40,41 and Slomianka and West.42