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. 2011 Feb;178(2):803–816. doi: 10.1016/j.ajpath.2010.10.034

Table.

Stereological Parameters Used for Cell Counting

Area Obj. B (μm2) H (μm) Sampling grid (μm) t (μm, mean ± SEM) Σ OD Σ CN CE
Hippocampus 100× 900 9 125 × 90 19.9 ± 0.8 291 655 0.042
Spinal cord 100× 2500 12 150 × 125 20.0 ± 0.3 183 157 0.082

B and H, base and height of the optical disectors; CE, average predicted coefficient of error of the estimated total numbers of neurons; Obj., objective lens power; t, measured average section thickness after histological processing; Σ CN, average number of counted neurons per specimen; Σ OD, average number of optical disectors used per specimen. For methodology, see Schmitz and Hof40,41 and Slomianka and West.42