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In situ RNA hybridization in different stages of CRC specimens for localization of SPAG9 gene expression. Representative H&E-stained sections of stage I, II, III, and IV are shown. Tissue sections from different CRC stages showed SPAG9 gene expression when probed with anti-sense riboprobe (chocolate brown color), but probing with sense riboprobe failed to show any reactivity. Original magnification, ×200; objective, 20×.
