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Immunohistochemical staining of CRC specimens for SPAG9 protein expression. Representative H&E- stained sections of stage I, II, III, and IV are shown. Serial tissue sections at different stages revealed SPAG9 cytoplasmic localization; however, no SPAG9 immunoreactivity was observed in ANCT and control colon specimens or with control IgG. The H&E-stained sections for stages I-IV are identical to those shown in Figure 2. Original magnification, ×200; objective, 20×.
