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. 2011 Mar;178(3):1009–1020. doi: 10.1016/j.ajpath.2010.11.047

Figure 6.

Figure 6

SPAG9 gene silencing significantly inhibited migration and invasion of human COLO 205 and HCT 116 cells. A: At 24 hours after transfection, the cells transfected with SPAG9 siRNA showed reduced migration through Transwell chambers, compared with control siRNA-transfected cells (observations based on three experimental triplicates). B: SPAG9 siRNA significantly impaired the ability of COLO 205 and HCT 116 cells to invade through Matrigel, compared with control siRNA. Data are reported as means ± SE of triple determinations. *P < 0.0001. C: Wound healing assay. Confluent COLO 205 and HCT 116 cells transfected with either SPAG9 siRNA or control siRNA were wounded, and images were acquired at 0, 12, 24, and 48 hours. These results are representative of three independent experiments performed in triplicate.