Skip to main content
. 2011 Mar;178(3):1009–1020. doi: 10.1016/j.ajpath.2010.11.047

Figure 7.

Figure 7

SPAG9 silencing inhibits the growth of colon tumors in vivo. A: Inhibition of subcutaneous solid tumor development in nude mice after treatment with SPAG9 siRNA, compared with control siRNA. At the end of the experiment, the tumors were surgically removed, weighed, and processed for immunohistochemical analysis. B: Tumor volume of nude mice treated with control siRNA (blue diamonds) or with SPAG9 siRNA (pink squares). C: Tumor weight in nude mice was reduced after treatment with SPAG9 siRNA, compared with control siRNA. Data are reported as means ± SE of 6 animals in each group. *P < 0.0001. D: Immunohistochemical analysis for SPAG9 protein expression in tumor specimens revealed strong SPAG9 immunoreactivity in tumors treated with control siRNA, compared with SPAG9 siRNA. Tumor sections probed for cellular proliferation marker Ki-67 demonstrated inhibition of cellular proliferation in tumors treated with SPAG9 siRNA, compared with control siRNA. In histograms, data are reported as positive reactivity of cells for SPAG9 protein or Ki-67, means ± SE. *P < 0.0001.