Figure 9.

Transplanted hepatocytes in the liver of mice with ALF. A: LacZ staining (cytoplasmic blue) in Rosa26 donor liver. B: Absence of LacZ staining in recipient NOD/SCID mouse liver. C and D: Representative examples of LacZ⁺ transplanted hepatocytes (blue) integrated in the mouse liver 14 days (C) or 3 months (D) after intrasplenic transplantation. Transplanted hepatocytes are arranged individually, agreeing with absence of their proliferation over time and indicating liver recovery occurred by proliferation of native hepatocytes. Original magnification, ×200. Scale bar = 100 μm. A hematoxylin counterstain was used.