Table 2.
Gene Expression Levels in NOD/SCID Mice Treated by Rif-Phen-MCT Versus Healthy NOD/SCID Mice
| Gene description | Gene symbol | NOD/SCID baseline | Time after drugs, days |
||
|---|---|---|---|---|---|
| 1 | 3 | 7 | |||
| Oxidative or metabolic stress | |||||
| Up-regulated | |||||
| Cytochrome P450, family 2, subfamily b, polypeptide 10 | Cyp2b10 | 1.0 | 1.2⁎ | 5.0 | 1.1⁎ |
| Cytochrome P450, family 3, subfamily a, polypeptide 11 | Cyp3a11 | 1.0 | 2.8 | 1.8⁎ | 3.4 |
| Glutathione peroxidase 2 | Gpx2 | 1.0 | 4.2 | 2.0 | 4.1 |
| Glutathione-S-transferase, μ3 | Gstm3 | 1.0 | 15.7 | 3.7 | 16.0 |
| Heme oxygenase (decycling) 1 | Hmox1 | 1.0 | 0.5⁎ | 5.2 | 0.5⁎ |
| Down-regulated | |||||
| Cytochrome P450, family 1, subfamily b, polypeptide 1 | Cyp1b1 | 1.0 | 0.3 | 0.7⁎ | 0.3 |
| Cytochrome P450, family 2, subfamily a, polypeptide 5 | Cyp2a5 | 1.0 | 0.3 | 0.1 | 0.3 |
| Cytochrome P450, family 2, subfamily b, polypeptide 9 | Cyp2b9 | 1.0 | 0.0 | 0.0 | 0.0 |
| Cytochrome P450, family 4, subfamily a, polypeptide 10 | Cyp4a10 | 1.0 | 0.1 | 0.1 | 0.1 |
| Cytochrome P450, family 4, subfamily a, polypeptide 14 | Cyp4a14 | 1.0 | 0.1 | 0.0 | 0.1 |
| Cytochrome P450, family 7, subfamily a, polypeptide 1 | Cyp7a1 | 1.0 | 0.1 | 0.0 | 0.1 |
| Flavin-containing monooxygenase 1 | Fmo1 | 1.0 | 0.8⁎ | 0.2 | 0.8⁎ |
| Flavin-containing monooxygenase 4 | Fmo4 | 1.0 | 0.4 | 0.2 | 0.4 |
| Heat shock | |||||
| Up-regulated | |||||
| Hsp 1 | Hspb1 | 1.0 | 3.5 | 27.4 | 3.6 |
| Hsp 1–like | Hspa1l | 1.0 | 2.0 | 0.4 | 2.4 |
| Hsp 8 | Hspa8 | 1.0 | 0.6⁎ | 2.4 | 0.0 |
| Down-regulated | |||||
| DnaJ (Hsp40) homolog, subfamily A, member 1 | Dnaja1 | 1.0 | 0.3 | 0.4 | 0.3 |
| Hsp 1 (chaperonin) | Hspd1 | 1.0 | 0.0 | 0.5 | 0.3 |
| Hsp 1 (chaperonin 10) | Hspe1 | 1.0 | 0.4 | 0.7 | 0.5 |
| Proliferation and carcinogenesis | |||||
| Up-regulated | |||||
| E2F transcription factor 1 | E2f1 | 1.0 | 2.4 | 0.7⁎ | 0.5⁎ |
| Down-regulated | |||||
| Cyclin D1 | Ccnd1 | 1.0 | 0.6⁎ | 0.1 | 0.5⁎ |
| Early growth response 1 | Egr1 | 1.0 | 0.3 | 9.9 | 0.2 |
| Growth arrest and senescence | |||||
| Up-regulated | |||||
| Cyclin-dependent kinase inhibitor 1A (p21) | Cdkn1a | 1.0 | 277.0 | 434.0 | 277.0 |
| DNA damage–inducible transcript 3 | Ddit3 | 1.0 | 1.2⁎ | 11.5 | 1.3⁎ |
| Growth arrest and DNA damage–inducible 45α | Gadd45a | 1.0 | 0.8⁎ | 74.0 | 1.0⁎ |
| Transformed mouse 3T3 cell double minute 2 | Mdm2 | 1.0 | 9.3 | 4.4 | 6.2 |
| Inflammation | |||||
| Up-regulated | |||||
| Chemokine (C-X-C motif) ligand 10 | Cxcl10 | 1.0 | 0.7⁎ | 3.1 | 0.9⁎ |
| Interleukin 6 | Il6 | 1.0 | 0.8⁎ | 4.5 | 0.6⁎ |
| Nitric oxide synthase 2, inducible, macrophage | Nos2 | 1.0 | 1.0⁎ | 5.7 | 0.8⁎ |
| Serine (or cysteine) peptidase inhibitor, clade E, member 1 | Serpine1 | 1.0 | 5.4 | 42.2 | 11.1 |
| Down-regulated | |||||
| Interleukin 1β | Il1b | 1.0 | 0.0 | 0.6⁎ | 0.1 |
| Interleukin 18 | Il18 | 1.0 | 0.4 | 0.5⁎ | 0.4 |
| DNA damage and repair | |||||
| Up-regulated | |||||
| Cyclin G1 | Ccng1 | 1.0 | 8.8 | 4.3 | 8.4 |
| Uracil DNA glycosylase | Ung | 1.0 | 1.3⁎ | 2.5 | 1.4⁎ |
| Down-regulated | |||||
| Ataxia telangiectasia mutated homolog (human) | Atm | 1.0 | 0.4 | 0.1 | 0.6⁎ |
| CHK2 checkpoint homolog (Schizosaccharomyces pombe) | Chek2 | 1.0 | 1.5⁎ | 0.2 | 1.7⁎ |
| RAD50 homolog (Saccharomyces cerevisiae) | Rad50 | 1.0 | 0.6⁎ | 0.1 | 0.7⁎ |
| X-ray repair complementing defective repair in Chinese hamster cells 4 | Xrcc4 | 1.0 | 0.5 | 0.2 | 0.5 |
| Apoptosis signaling | |||||
| Up-regulated | |||||
| Annexin A5 | Anxa5 | 1.0 | 2.3 | 1.3⁎ | 2.8 |
| Bcl2-associated X protein | Bax | 1.0 | 3.1 | 0.6⁎ | 3.7 |
| Bcl2–like-1 | Bcl2l1 | 1.0 | 0.3 | 2.1 | 0.7⁎ |
| Down-regulated | |||||
| Fas ligand (TNF superfamily, member 6) | Fasl | 1.0 | 0.1 | 0.2 | 0.1 |
| TNF (ligand) superfamily, member 10 | Tnfsf10 | 1.0 | 0.2 | 0.1 | 0.3 |
| TNFRSF1A-associated via death domain | Tradd | 1.0 | 0.5⁎ | 0.2 | 0.5⁎ |
NOD, natural-onset diabetes; SCID, severe combined immunodeficiency; Rif, rifampicin; Phen, phenytoin; MCT, monocrotaline; NS, not significant; Hsp, heat shock protein; TNF, tumor necrosis factor.
⁎
Statistically not significant