Figure 5.

CCR2 mediates the early accumulation of Ly-6C^high monocytes in the peripheral blood and spleens of mice that clear cryptococcal lung infection. Organ-specific leukocytes were obtained from CCR2^+/+ mice (wild type, BALB/c) or syngeneic CCR2^−/− mice that were either uninfected (designated D0) or at 7, 14, 21, and 28 dpi with C. neoformans. Leukocytes were stained with specific antibodies and analyzed by flow cytometric analysis to identify Ly-6C^high monocytes (defined as CD45⁺ Ly-6C^high CD11b⁺ cells). A through C: Kinetic analysis of Ly-6C^high monocytes during cryptococcal lung infection in peripheral blood mononuclear cells (PBMCs) (A), bone marrow (B), and spleen (C). Data in A are the frequency of Ly-6C^high monocytes within PBMCs. Data in B and C are total numbers of Ly-6C^high monocytes in each tissue; the mean ± SEM of three to seven mice was assayed individually per point; CCR2^+/+ mice, black bars; CCR2^−/− mice, gray bars. *P < 0.05 by unpaired Student's t-test between groups at the same point.