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. 2011 Jan;13(1):85–92. doi: 10.1016/j.jmoldx.2010.11.004

Figure 2.

Figure 2

Analytic sensitivity of Δ-PCR for BCL2-IGH translocation. Genomic DNA (250 pg, or 40–50 cell equivalents) from cell lines SU-DHL-6 (A), SU-DHL-4 (B), and SU-DHL-16 (C) (positive control for MBR1, MBR2, and mcr2 breakpoints, respectively) was subjected to multiplex Δ-PCR and analyzed by electrophoresis (ABI 3100 system, Applied Biosystems, Foster City, CA) with 45-second electrokinetic injection. Blue peaks are Δ-PCR products (Δ numbers indicate amplicon size difference in base pairs) and red peaks are size markers. The presence of two peaks with size difference of Δ18 ± 1 bp, Δ24 ± 1 bp, and Δ33 ± 1 bp indicates BCL2-IGH translocation with breakpoints at MBR1, MBR2, and mcr2, respectively.