Table 1.
Paired Forward Primers Used in Δ-PCR for Detection of BCL2-IGH Translocation
| Forward primer⁎ | Sequences | Primer ratio | Reference |
|---|---|---|---|
| MBR1 (18 bp) | |||
| MBR1int | 5′-CTATGGTGGTTTGACCTTTAGAGAG-3′ | 1 | 16 |
| MBR1ext | 5′-TGACCAGCAGATTCAAATCTATGG-3′ | 1 | 13† |
| MBR2 (24 bp) | |||
| MBR2int | 5′-ACTCTGTGGCATTATTGCATTATATACC-3′ | 2 | 13† |
| MBR2ext | 5′-CAGCCTTGAAACATTGATGGA-3′ | 1 | 35† |
| mcr1 (39 bp) | |||
| mcr1int | 5′-CTTGCAGGGTCTTTAAGCAGCA-3′ | 1 | |
| mcr1ext | 5′-CATAGAGCAAGCGCCCAATAAATA-3′ | 4 | 13† |
| mcr2 (33 bp) | |||
| mcr2int | 5′-GTACTTCAGAAGGAGGAACGTCCT-3′ | 1 | |
| mcr2ext | 5′-TGAATGCCATCTCAAATCCAA-3′ | 4 | 13 |
⁎
Combined with the reverse primer VLJH (5′-6FAM-GTGACCAGGTNCCTTGGCCCCAG-3′). The expected size difference between products amplified with each of the forward primers paired with the reverse primer is given in parentheses.
†
With modification.