Table 3.
Primary Antibodies Used for Immunohistochemistry
| Targeted epitope | Antibody type | Host⁎ | Clone | Manufacturer† | Catalog no. | Dilution |
|---|---|---|---|---|---|---|
| Human melanosome | IgG1, κ | M | HMB-45 | DAKO | M0634 | 1:50 |
| Melan A | IgG1, κ | M | A103 | DAKO | M7196 | 1:100 |
| S100B | IgG | R | — | DAKO | Z0311 | 1:900 |
| P63 (pan) | IgG2a | M | 4A4 | DAKO | M7247 | 1:50 |
| P63-alpha | IgG | R | — | CST | 4892 | 1:20 |
| Keratin-15 | IgG2a | M | MS-1068 | TFS | LHK15 | 1:150 |
| Keratin-19 | IgG1 | M | 4A36 | USB | C9097-24B | 1:150 |
| Ki-67 | IgG | R | — | TFS | RB-1510R7 | 1:200 |
| Connexin 43 | IgG1, κ | M | CX-1B1 | Zymed | 13-8300 | 1:100 |
| Isotype | IgG1 | M | — | DAKO | X0931 | 1:100 |
| Isotype | IgG | R | — | DAKO | X0903 | 1:900 |
⁎
M, mouse; R, rabbit.
†
DAKO, DakoCytomation; CST, Cell Signaling Technology; TFS, Thermo Fisher Scientific; USB, United States Biological; Zymed, Zymed Laboratories.