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. 2011 Mar;178(3):983–988. doi: 10.1016/j.ajpath.2010.12.007

Figure 3.

Figure 3

Evaluation of AIRE in postnatal hair cycling and hair follicle development. Individual or overlaid fluorescent images for DAPI (blue), K17 (green), or AIRE (red) are shown in adult telogen and anagen hair follicles (A) and 16-week-old human fetal scalp (B). A: AIRE expression in the outer root sheath of both telogen (left) and anagen (right) hair follicles, with some colocalization with K17 (subcellular close-up). White boxes indicate areas of magnification from ×10 to ×63 (red arrow) and from ×63 to subcellular close-up (blue arrow). B: AIRE expression in the nonfollicular epidermis and developing follicular pegs, but not the primary hair germ in human fetal scalp. White dashed lines in the AIRE channel delineate the epidermal/dermal border. K17 expression shown in both primary germ and follicular peg; subcellular magnification with AIRE colocalization (bottom). Rabbit isotype control (right) is included to show negative staining comparison to primary germ for AIRE. White box indicates area of close-up. Representative colocalization scatter plots with associated PCCs are shown corresponding to the ×63 image indicated in A and B. Scale bar = 150 μm for ×10, 25 μm for ×63, and 10 μm for subcellular close-ups shown in A and B.