Figure 1.

pK5-CDK6 construct and PCR screening. (A) Diagram of the K5CDK6 construct. (B) PCR amplification of DNA extracted from mouse tails. The CDK6 transgene was amplified, resulting in a 850-bp product. (C) Expression and quantification of CDK6 in each of the four mouse lines (A–D). (D) CDK6 expression was detected by immunofluorescence analysis in paraffin cross sections of wild-type (WT) and K5CDK6(H) mice. MW, molecular weight.