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. 2011 Mar;178(3):1080–1091. doi: 10.1016/j.ajpath.2010.11.072

Figure 5.

Figure 5

Induction of HSP70 after administration of geranylgeranylacetone (GGA). A: Western blot analysis showed increased HSP70 protein level in the attached retina with GGA administration. There was no change in the HSP70 protein level after administration of GGA with quercetin and vehicle. B–D, F–N: Immunofluorescent analysis for HSP70. In the vehicle, expression of HSP70 was faintly seen in the inner segment (IS). In contrast, the IS of the GGA group showed dense expression of HSP70. In the GGA with quercetin group, expression of HSP70 was similar to vehicle. E: Relative protein expression ratio of HSP70 at 0, 1, 3, and 7 days after retinal detachment (RD) in each group. The protein expression level of HSP70 was normalized by the normal group. In the GGA group, the expression was already up-regulated before RD (*P < 0.01). Equal quantities of protein were loaded in all lanes, and representative exposures of enhanced chemiluminescence detection of the immunoreactive proteins are shown. Each column represents the mean ± SD of six independent experiments. Scale bars = 100 um. GCL, retinal ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; Q, quercetin.