Figure 7.

HSP70 induce Akt activation after retinal detachment (RD). A–C: Western blot analyses show the dynamics of protein expression of phosphorylated Akt (pAkt) and total Akt in each group. D–F: Relative protein expression ratio of pAkt 1, 3, and 7 days after RD in each group. The expression protein of pAkt was normalized by the normal group (attached retina). Activation of Akt was suppressed with time-dependent manner (*P < 0.01; **P < 0.05). Equal quantities of protein were loaded in all lanes, and representative exposures of enhanced chemiluminescence detection of the immunoreactive proteins are shown. Each column represents the mean ± SD of four independent experiments. G–Q: Immunofluorescent analysis for pAkt in retina of normal, vehicle, quercetin, geranylgeranylacetone (GGA), GGA with quercetin, and HSP^−/− mice groups at 1 and 3 days after RD. In the normal (attached retina), pAkt were rarely expressed in the whole layer. In the vehicle group, expression of pAkt was observed in the retinal ganglion cell layer (GCL) and outer nuclear layer (ONL). In the quercetin and HSP^−/− mice group, pAkt were faintly expressed in the GCL at day 1; however, expression pattern was similar to the normal group at day 3. In the GGA group, expression of pAkt was remarkably observed in the GCL and ONL. On the other hand, expression of pAkt was similar to vehicle in the GGA with quercetin (Q). Each column represents the mean ± SD of four independent experiments. Scale bars = 100 um. INL, inner nuclear layer; WT, wild type.