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. 2011 Mar;178(3):989–996. doi: 10.1016/j.ajpath.2010.12.003

Figure 3.

Figure 3

Profile of mesenchymal markers in circulating tumor microemboli (CTM) and circulating tumor cells (CTCs) from lung cancer patients. Blood samples from lung cancer patients were subjected to ISET filtration and CTM/CTCs were profiled for vimentin or neural cadherin (see Materials and Methods). A: Vimentin staining in lung cancer patients' CTM (left and middle panel), CTCs (upper and lower right panel), with white blood cells and cultured DMS114, used as positive and negative staining controls. White blood cells are discriminated from CTM/CTCs by virtue of their smaller size and distinct nuclear morphology. B: Heterogeneous staining for neural cadherin in both CTM (left and middle panel) and CTCs (upper and lower right panel). Cultured H524 SCLC cells were used as the positive control and white blood cells were negatively stained and distinguished from tumor cells by virtue of their smaller size and distinct nuclear morphology. Images shown are representative from the patient cohort (see Table 1).