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. 2011 Mar;178(3):1309–1315. doi: 10.1016/j.ajpath.2010.11.069

Figure 2.

Figure 2

T2-reactive PrPres fragments in the cerebellum from the sCJD-MM1+2 patient. A: Imunohistochemistry using the anti-PrP antibody #71 showed that perivacuolar PrP deposition was restricted to within the cerebral cortices in the sCJD-MM1+2 patient (cases 45; ×40, insets × 200). The sCJD-MM1 patient showed only synaptic-type PrP deposition (case 47; ×40). G = gray matter; W = white matter. B: In the conventional Western blot analysis using the 3F4 antibody, faint type 2 PrPres bands (arrowheads) were detected in the cerebrums from the sCJD-MM1+2 patients (cases 2, 5, 45, and 55) but not in the cerebellum (case 45 CE). In case 44, although neuropathological examination showed focal perivacuolar PrP deposition, the conventional Western blot analysis failed to detect type 2 PrPres. C: T2-reactive PrPres fragments could be detected also in the cerebellum of the sCJD-MM1+2 patient (case 45 CE). D: The signal intensities of the 3F4-reactive PrPres fragments (white bars) or those of the T2-reactive PrPres fragments (gray bars), and the T2/3F4 ratios (black bars). The mean signal intensities of PrPres in the sCJD-MM2 brain (case 16) were assigned as 100/mm2 in each experiment. Data are expressed as mean ± SEM (n = 3).