Figure 3.

T2-reactive PrP^res fragments in the cerebellums from the sCJD-MM1 patients. A: In the conventional Western blot analysis using the 3F4 antibody, PrP^res was not detected in the cerebellum of the sCJD-MM2 patient (case 16 CE). B: The T2-reactive PrP^res fragments could be detected in the cerebellums from the sCJD-MM1 patients (cases 47 CE and 49 CE), but not in the cerebellum from the sCJD-MM2 patient (case 16 CE). C: The signal intensities of the 3F4-reactive PrP^res fragments (white bars) or those of the T2-reactive PrP^res fragments (gray bars), and the T2/3F4 ratios (black bars). The mean signal intensities of PrP^res in the sCJD-MM2 cerebrum (case 16) were assigned as 100/mm² in each experiment. Data are expressed as mean ± SEM (n = 3).