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. 2001 Mar 13;98(6):3600–3605. doi: 10.1073/pnas.051511398

Figure 1.

Figure 1

CaT-L. shows Ca2+-dependent inactivation and binds calmodulin. (A) Representative current recorded during a voltage step to −100 mV. At least two inactivation time constants can be distinguished in 30 mM [Ca2+]o. (B) Current recorded in 30 mM [Ba2+]o. Note that activation is delayed, the initial fast inactivation component is missing, and currents only show a very slow second component of inactivation. (C) 35S-labeled CaT-L binds to CaM-agarose in the presence, but not absence of Ca2+ (Left). GST-fusion proteins of CaT-L were constructed according to the schematic drawing (Right). aa, amino acid residues. (D) Binding of [35S]CaM to different CaT-L GST-fusion proteins in the presence (+) or absence of calcium.