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. Author manuscript; available in PMC: 2012 Mar 8.
Published in final edited form as: Biochemistry. 2011 Jan 31;50(9):1474–1482. doi: 10.1021/bi1013535

Table 1.

Equivalent positions in the AlaRS editing active site as inferred from multiple sequence alignmentsa.

Predicted contact
to the Ser-
tRNAAla substrate		 P. horikoshii
AlaX		 E. coli
Full length
AlaRS		 H. sapiens
Full length
AlaRS		 P.
horikoshii
Full
length
AlaRS		 A. fulgidis
Full length
AlaRS
Main chain
α-amino group His13 His568 His609 His617 His604
α–carboxyl group Asn 114 Glu664 (Ala) Glu721 Gln715 Gln701
α-carboxyl group Cys116 Cys666 (Ala) Cys723 Cys717 Cys703
α-carboxyl group Thr33 Ser587 Ser627 Ser636 Ala623
α- carbon Leu12 Thr567 (Gly) Thr608 Thr616 Thr603
Side chain
contacts hydroxyl R-group Thr30 Gln584 (His, Asn, Ala) Gln624 Gln633 Gln620
contacts hydroxyl R-group) Asp92 Phe645 Phe686 Gln695 Gln682
“Sticky mouse” linked mutation Ile127 Ile677 (Glu) Ala734 Val728 Ile714
a

Substitutions introduced into the residues of interest examined in this study are indicated in parenthesis.