Supplemental Figure 2.

Testing of the constructs of recombinant adeno-associated viral vectors expressing the shRNA against FKRP. A: Reduction of the FKRP mRNA transcript by transfected pAAV-U6-shFKRP (2 μg) in the Hepa 1-6 cell line (n = 9, mean ± SD, *P < 0.001, #P < 0.005). B: Overexpressed mouse FKRP-EYFP fusion protein in 293 cells was partially knocked down by pAAV-U6-shFKRP2 + 5. Transfected cells were observed under fluorescent microscope. Scale bar, 100 μm.